Diisopropylfluorophosphate ( DFP ) of MDCK Cells InhibitsRicin - induced Apoptosis
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چکیده
Diisopropylfiuorophosphate {OFP), a general serine protease inhibitor, inhibited the DNA fragme"tatioll and cell death in MDCK cells treated with ricin, modeccin, Ilseudomonas toxin, or dipbtberia toxin. Atrypsin-like serine protease inhibitor, IVLtosyl・L-lysine chleromethyl ketone (TLCK) also prevented ricin-induced DNA fragmentation and cel] death, albeit less effectiyely than DFP. Microscopic observation showed that the morphological changes of MDCK cells illduced by ricin were preyented by DFP. DFP did net affect the binding, internaiizatioll, or subsequent ex-
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